Rapid, specific assay for plasma cortisol by competitive protein binding.
نویسندگان
چکیده
We describe a modified competitive protein-binding method for assay of plasma cortisol. Plasma samples are deproteinized by dilution with an ethanol/phosphate buffer, followed by heating at 100 degrees C for 2 min. Horse serum is used as the source of transcortin. Free radioactivity is separated from the protein-bound component by partition into liquid scintillation counting within 60 min. The assay has better specificity and precision than a competitive protein-binding assay in which ethanol extraction and Florisil adsorbent are used, and results correlate well with those of a specific radioimmunoassay method.
منابع مشابه
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عنوان ژورنال:
- Clinical chemistry
دوره 21 10 شماره
صفحات -
تاریخ انتشار 1975